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Assessing Antigens immunogenicity

• Cloning of the cDNA in a suitable expression vector
• Engineering of the antigen to enhance its immunogenicity (e.g. codon optimization, deletions, fusion with immuno-enhancing sequences)
• Determination of immunologic breakpoint in tolerant preclinical models (mouse, rat)
• Measurement of the immune response (T cell, antibodies, innate immunity)

Example: evaluation of immunogenicity of a modified antigen

Workflow

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Comparing immunogenicity of vaccination platforms and adjuvants

• DNA electro-gene transfer
• Adenoviral vectors
• Dendritic cells
• Peptides
• Proteins
• TLR agonists
• Immunomodulatory Abs

Example: comparison of DC vs DNA-EGT/Ad for antigen X in mice

New adjuvants/immunomodulators validation workflow