Generation, optimization and validation of ELISA kits

  • Definition of reagents to be used
  • Preliminary assessment of assay characteristics
  • Optimization of working range, sensitivity
  • Establishment of assay parameters such as limit of detection, limit of quantitation
  • Evaluation of matrix effects, sample performance
  • Assessment of reproducibility
  • SOPs and protocols

Example: Set up of a kit to quantify a human biomarker

elisa kit graph


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Takis is a biotech dedicated to cancer research. Our primary objective is the development of innovative therapies based on the principle that our immune system can be “instructed” to recognize the tumor and reject it as a foreign entity.