The Mannan Induced Psoriatic Arthritis (MiP)

The mannan (Saccharomyces Cerevisiae) induced PsA model (MiP) in mice is a newly developed model in which both skin lesions resembling those seen in Ps as well as the joint inflammation of PsA can be found. Disease is mediated by TNF-a produced by macrophages which leads to T cell activation and IL-17A production. The increased level of IL-17A recruit neutrophils to joint and skin and results in an inflammatory response. Repeated mannan injections (every 7th day) give rise to a chronic relapsing disease.

Onset of joint and skin inflammation is seen within a few days after the mannan injection and slowly heals out after one week if not reactivated by a second mannan injection.

STUDY ENDPOINT

Histopathologic analysis
Macroscopic scores (skin and arthritis scores)
Ear epidermal thickness
Blood collections and analysis
Stool collections (NGS analysis of microbiota)
Tissue collections
Cytokine analysis and other inflammatory markers (IL-6, IL-17a, TNFα, TGFβ)

VALIDATION DATA

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Arthritis and skin scores in mannan-treated mice compared with not-treated mice.

Negative CTRL

Representative H&E staining of ears section, inflammation scoring and epidermal ear thickness

PsA mannan 20mg

PAWS PICTURES

BELLY PICTURES

EARS PICTURES

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