TNBS-Induced Colitis Model

Haptinizing agent 2,4,6-trinitrobenzene sulfonic acid (TNBS) dissolved in ethanol and intrarectally administered to a mouse through a special catheter is a suitable method to induce colitis. Ethanol is required to break the mucosal barrier, whereas TNBS is believed to haptenize colonic autologous or microbiota proteins rendering them immunogenic to the host immune system. CD4+ T cells are suggested to play a key role in inducing colitis in this mouse model. The acute TNBS colitis model is usually induced by a single intrarectal administration and then observed for 3 days.


STUDY ENDPOINT
  • MPO levels
  • DAI and Macroscopic scores
  • Cytokine analysis and other inflammatory markers (TNFa, IL-1b, SAA, KC)
  • Blood collections and analysis (Fe2+ and Hemoglobin)
  • Stool collections (NGS analysis of microbiota)
  • Tissue collections
  • Intestinal permeability (FITC-Dextran)
  • Histopathologic analysis
VALIDATION DATA
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Body weight loss and colon shortening in 2% TNBS-treated mice compared with mesalazine and vehicle ethanol-treated mice.

Representative H&E staining of Colon section and colon scoring

Dextran Sulfate Sodium-Induced Colitis Model

Dextran Sulfate Sodium-Induced Colitis Model

TNBS-Induced Colitis Model

TNBS-Induced Colitis Model

Ligature-Induced Periodontitis Model

Ligature-Induced Periodontitis Model

Diet-Induced Obesity Model

Diet-Induced Obesity Model

Human Tumor Xenograft Model

Human Tumor Xenograft Model

ANIMAL FACILITY

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Takis is a biotech dedicated to cancer research. Our primary objective is the development of innovative therapies based on the principle that our immune system can be “instructed” to recognize the tumor and reject it as a foreign entity.