TK-Neo is a individualised therapeutic cancer vaccine against tumour specific antigens, called neoantigens, arising from somatic gene mutations in malignant cells during neoplastic transformation. The tailored vaccine is intended for use as therapeutic vaccination in patients with locally advanced or metastatic solid tumours.

The process

  • Tumor Biopsies are taken from patient
  • RNA and DNA are sequenced by Next Generation Sequences
  • Neoantigens are identified through a pipeline called NARciso
  • Codon Optimized Vaccine is synthesized in Takis Laboratories
  • 100-150mg DNA plasmids are manufactured in GMP conditions
  • The entire process is predicted to be back to the patient in 6 weeks

Where we are:

  • Preclinical studies completed
  • Biomarker assay under advanced development
  • Specialty Oncology Centers / KOLs identified and ready to start

Cancer Targets

  • Melanoma
  • Lung Cancer


The pipeline of neoantigen cancer vaccine production, for mouse studies (left side and human studies (right side). 1. Tumor and normal tissue are collected and subjected to (2) exome sequencing and RNAseq analysis for the tumor samples. 3. expressed non-synonymous mutations are then further selected according to binding to predictive algorithms and incorporated in a vaccine vector or delivered as peptides with adjuvants.
Personalized NCV in the context of current immunotherapy, the three dimensions are defined by tumor infiltrating lymphocytes (TILs), mutational load (ML) and tumor heterogeneity (TH). Patients in the lower right panel (TILML+TH+) may benefit from Neoantigen cancer vaccine (NCV) approach whereas patients in the upper right panel (TILs+ML+TH) respond more to immune checkpoint inhibitors (ICI)
Aurisicchio L, Pallocca M, Ciliberto G, Palombo F.
J Exp Clin Cancer Res. 2018 Apr 20;37(1):86. doi: 10.1186/s13046-018-0751-1

Follow Us on Linkedin

Takis is a biotech dedicated to cancer research. Our primary objective is the development of innovative therapies based on the principle that our immune system can be “instructed” to recognize the tumor and reject it as a foreign entity.